AimAngiopoietin-like proteins 3/4/8 (ANGPTL3, ANGPTL4 and ANGPTL8) are associated with the regulation of lipid metabolism, while lipid metabolism is essential for metabolism homeostasis. In this study, we focused on these proteins to understand their role and involvement in Non-alcoholic fatty liver disease (NAFLD) amelioration after sleeve gastrectomy. MethodsANGPTL3, ANGPTL4 and ANGPTL8 expression were assessed in liver, white adipose tissue (WAT), brown adipose tissue (BAT) and muscle of NAFLD male Sprague Dawley rats with diet induced obesity (DIO) subjected to sham and sleeve gastrectomy (SG) surgery. ResultsThe mRNA expression of ANGPTL8 was significantly increased in the liver tissue of SG group whereas ANGPTL4 was significantly decreased. The expression of ANGPTL3 remained unaltered. In WAT, the expression of ANGPTL4 significantly increased in the SG group, however there was no change observed in ANGPTL8. In BAT, the expression of ANGPTL4 and ANGPTL8 remained unaltered. In muscle, the expression of ANGPTL4 was higher in DIO-Obese and Sham animals compared to healthy control and SG group. ConclusionThe study highlights the relationship between the expression of the ANGPTL proteins and their importance in regulating lipid metabolism. Together, ANGPTL8 is involved in the resolution of NAFLD after sleeve gastrectomy through the mechanism of lipogenesis inhibition.